Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample preppers. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software. There are 2 types of worksheets between which you can toggle worksheet view button. Cst cytometer setup and tracking is a module that runs within bd facsdiva. Bd facsdiva software is a flexible data acquisition and analysis package specifically designed for digitalbased flow cytometers. With histogram you can look at the distribution of the cell intensities very easily. Cytometry win upgrades on diva computers edited subject. Cytometer function is controlled by bd facsdiva software. The software uses flexible features to simplify acquisition, including experiment templates, userdefinable experiment layouts, and automated compensation calculation. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password. Cells were sorted on high purity setting at no more than 5000 eventss using a 70 m nozzle at 55 psi with a 90 hz.
Application modules for hiv immunophenotyping, stem cell enumeration and hlb27. Bd facsdiva bead lot files resources software bd biosciences. The bd lsr ii is equipped with three lasers 405, 488 and 635 nm and can handle advanced multicolor applications. Characterization of a cytometer this part summarizes the activities required for the initial measurements for cytometer characterization, including creating a standard configuration. The software uses flexible features to simplify acquisition, including experiment templates and automated compensation calculation. Bd facsdiva software quick reference guide for the bd lsr ii. New look for workspace windows the bd facsdiva software has been updated to display in the titles of the browser, the inspector, the cytometer window, and the worksheet window, the active component for that window. This guide contains instructions for using bd facsdiva software version 6 with bd facscanto and bd facscanto ii systems equipped with the bd facs loader option. Therefore, new data continuously displaces old data and is kept in memory only for a short amount of time approximately 0. The basic 2dimensional dot plots and density plots are naturally supported.
If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Open bd facsdiva software and use for analysis as normal if you have any questions feel free to contact me. Features technical bulletins standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software bd biosciences march 2012 part 1. Bd facsdiva software resourcestools beadlotfiles bd.
Bd biosciences, a segment of bd becton, dickinson and company, today announced the introduction of bd facsdiva 6. Reading facsdiva data files flowjo v10 documentation. Facsdiva is bd biosciences next generation data acquisition and analysis program and operates in the pc windows environment on the facsaria, facscanto, lsrii and fortessa. Bd facs canto ii flow cytometer quick user guide v. Refer to new features in getting started with bd facsdiva software for details. Tm, use the pe channel for reporter and the apc channel for beads classification. How to use experiment layout in facsdiva raffaello cimbro. The bd protocol discusses what to do in these instances. In this guide, you will find a description of bd facsdiva software features specific to the hts option. Bd facsdiva software reference manual for version 8. Figure 11 bd facscanto ii flow cytometer the bd facscanto ii flow cytometer consists of a fluidics subsystem, an optics subsystem, and an electronics subsystem. User are asked to contact bd customer support center at 18772328995 if they have any additional questions. Open bd facsdiva software and use for analysis as normal if you have any questions feel free to. Access bd lsr ii online help from the help menu of bd facsdiva software.
Bd facsdiva software contains vxworks as embedded software runtime module. Standardizing application setup across multiple flow. Not for use in diagnostic or therapeutic procedures. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. Aug 31, 2017 download sentinel protection installer v7. Bd bd becton, dickinson and company, a leading global medical technology company has announced the availability of flowjo software version 10. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. The software adds new analysis capabilities and enhances integration with bd facsdiva software, making it easier for users to analyze. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis. This automated package will help scientists attain the best performance and most consistent data from bd flow cytometry systems.
Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk. Rightclick the original file and choose properties 5. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Bd facsdiva software is a flexible data acquisition and analysis package designed for bd facs brand digital flow cytometers.
The module must be run using cst beads, from the correct bead lot 6. Sophie novault bd facs diva software research institut. Workflow overview the following figure shows the steps for daily workflow using bd facsdiva software. Refer to the information supplied by the manufacturer for applicationspecific limitations. Bd biosciences will release a version of bd facsdiva software to correct this problem. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. The runtime module was developed by a third party and we are obligated to notify our customers about the limitations for use. Biexponential scaling can be used to show all events on plots, even those with negative values. This part of the guide tmapplies to bd digital flow cytometers using facsdiva software version 6. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Bd lsr ii, bd facscanto, and bd facscanto ii flow cytometers modified with the hts can acquire samples from plates or tubes. Displays data from one tube at a time uses the same set of plotsgates to analyze any tube in an experiment.
The online help installed with your bd facsdiva software contains the same text as that in the documents listed below, enhanced with features like full text search and related topic links. Interference there are no known incompatibilities with bd facscanto clinical. Bd facslink lis interface solution with bd facsdiva software elearning course developed in collaboration with data innovations, a leading middleware provider experienced in clinical applications, this laboratory information system lis interface solution allows the direct transfer of information between bd software and an lis, reducing errors commonly caused by manual transcriptions. Bd facsdiva electronics create an enormous amount of data, about 1,000 mb of data every minute. The beads allow the software to automatically characterize, track, and report measurements of supported bd digital flow cytometers. Select exit only, and click launch bd facsdiva software, enter your user name and password, and click ok.
As the name suggests, it is used to setup certain components of the system eg. This problem can occur on bd facsdiva versions b 4 and b 4 when combined with a specific computer system configuration. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample preppers bd facsdiva software v6. Under attributes, unclick readonly, then click ok 6. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. If the data was compensated during acquisition, this compensation matrix is included in the data file in flowjo, it is referred to as the acquisition matrix. This problem has not been reproduced in other computer systems or in other bd facsdiva software versions distributed by bd biosciences.
May 17, 20 the bd protocol discusses what to do in these instances. Firsttime users of bd facsdiva software should read. Using application settings to standardize flow cytometry. The bd facsdiva software reference manual includes instructions or descriptions for installation and setup, workspace components, acquisition controls, analysis tools, and data management. Up to eight different fluorescent probes can be measured simultaneously. It can be accessed from the bd facsdiva software help menu help literature reference manual, or by doubleclicking the shortcut on the desktop. Software operating system microsoft windows 7 bd facsdiva software version 8. Bd facscanto ii instructions for use manual pdf download. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. Ellen meinelt, mervi reunanen, mark edinger, maria jaimes, alan stall, dennis sasaki, joe trotter.
Bd lsr ii online help topics are compiled from the following sources. Dec 30, 2017 this video will show you how to install bd facsdiva 8. Expand the specimen and make the tube active by clicking the arrow next to the tube. Description bd cytometer setup and tracking beads are for research use only with bd facsdiva software v 6. To process signals from a single event passing sequentially through two or. Optimizing with bd facsdiva software to optimize cytometer settings in bd facsdiva software. A free powerpoint ppt presentation displayed as a flash slide show on id. Bd facsdiva software resources software bd biosciences. Allow the instrument to connect to the software if necessary. Setup procedure for bd facsariatm, facscantotm and lsr series. The markers act both as regions and statistical markers.
Any use of products other than the permitted use without the express written authorization of becton, dickinson and company is strictly prohibited. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample. If you are not familiar with the windows operating system, see the documentation provided with your computer. Before starting your daily workflow, ensure that your labs software administrator has performed all the necessary tasks. This guide contains instructions for using bd facsdiva software version 6. The letter also stated that bd biosciences rep will contact the users when the new software is available. Becton dickinson bd facsdiva software is a technology used for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows in a laboratory and healthcare setting. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Machine does not connect on switching on and booting up. Dealing with a clog on the bd lsr ii flow cytometer duration.
159 101 1571 909 890 359 437 817 1136 1454 677 1135 657 941 1185 1011 1579 426 1252 67 628 1275 329 911 1217 632 243 201 1408 1381 1049 1118 1479 381 201 724 894